The Effect of Hypoxic Exercise Combined with Crocodile Blood Supplementation on Aerobic Capacity and Hematological Variables in Athletes
DOI:
https://doi.org/10.17309/tmfv.2024.1.12Keywords:
crocodile blood supplement, hematological, aerobic capacity, hypoxic training, amateur male soccer playersAbstract
Study purpose. This study aimed to compare the effects of hypoxic exercise combined with crocodile blood supplementation on hematological and maximum oxygen consumption parameters in soccer players.
Materials and methods. This study included 39 male soccer players aged 21.69 ± 1.98 years who were randomized to three groups. All groups performed the same training program, which comprised treadmill exercise at 70%–75% of maximum heart rate for 7 weeks with sessions of 30 min/day and 3 days per week. The first group was the control placebo group (CG, n = 13), the second group received one capsule per day (60 mg) of a freeze-dried crocodile blood supplement (SUP, n = 13), and the third group received the freeze-dried crocodile blood supplement and performed the training program under hypoxic conditions (H-SUP, n = 13, FiO2 = 16.3%). A hematological evaluation was conducted and maximal oxygen uptake (VO2max) was measured using the Bruce protocol treadmill test.
Results. The H-SUP group demonstrated significantly higher erythropoietin (EPO) levels (14.40 ± 2.41 mIU/mL) compared to the SUP group (11.50 ± 2.08 mIU/mL) and CG (12.01 mIU/mL) after the intervention. From pre- to post-intervention, VO2max significantly increased in the H-SUP (Pre: 45.63 ± 4.75, Post: 49.33 ± 5.81) and SUP groups (Pre: 44.59 ± 4.75, Post: 47.30 ± 5.68) but not in the CG.
Conclusions. This study reveals that a combination of freeze-dried crocodile blood supplementation and hypoxic exercise causes hematological alterations, particularly the activation of EPO secretion, and increases VO2max in soccer players.
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Ponsot, E., Dufour, S. P., Zoll, J., Doutrelau, S., N’Guessan, B., Geny, B., . . . Richard, R. (2006). Exercise training in normobaric hypoxia in endurance runners. II. Improvement of mitochondrial properties in skeletal muscle. J Appl Physiol (1985), 100(4), 1249–1257. https://doi.org/10.1152/japplphysiol.00361.2005 DOI: https://doi.org/10.1152/japplphysiol.00361.2005
Czuba, M., Waskiewicz, Z., Zajac, A., Poprzecki, S., Cholewa, J., & Roczniok, R. (2011). The effects of intermittent hypoxic training on aerobic capacity and endurance performance in cyclists. J Sports Sci Med, 10(1), 175–183. https://www.ncbi.nlm.nih.gov/pubmed/24149312
Powell, F. L., & Garcia, N. (2000). Physiological effects of intermittent hypoxia. High Alt Med Biol, 1(2), 125–136. https://doi.org/10.1089/15270290050074279 DOI: https://doi.org/10.1089/15270290050074279
Katayama, K., Sato, K., Matsuo, H., Ishida, K., Iwasaki, K., & Miyamura, M. (2004). Effect of intermittent hypoxia on oxygen uptake during submaximal exercise in endurance athletes. Eur J Appl Physiol, 92(1-2), 75–83. https://doi.org/10.1007/s00421-004-1054-0 DOI: https://doi.org/10.1007/s00421-004-1054-0
Roels, B., Millet, G. P., Marcoux, C. J., Coste, O., Bentley, D. J., & Candau, R. B. (2005). Effects of hypoxic interval training on cycling performance. Med Sci Sports Exerc, 37(1), 138–146. https://doi.org/10.1249/01.mss.0000150077.30672.88 DOI: https://doi.org/10.1249/01.MSS.0000150077.30672.88
Hamlin, M. J., Marshall, H. C., Hellemans, J., Ainslie, P. N., & Anglem, N. (2010). Effect of intermittent hypoxic training on 20 km time trial and 30 s anaerobic performance. Scand J Med Sci Sports, 20(4), 651–661. https://doi.org/10.1111/j.1600-0838.2009.00946.x DOI: https://doi.org/10.1111/j.1600-0838.2009.00946.x
Bonetti, D. L., Hopkins, W. G., & Kilding, A. E. (2006). High-intensity kayak performance after adaptation to intermittent hypoxia. Int J Sports Physiol Perform, 1(3), 246–260. https://doi.org/10.1123/ijspp.1.3.246 DOI: https://doi.org/10.1123/ijspp.1.3.246
Hendriksen, I. J., & Meeuwsen, T. (2003). The effect of intermittent training in hypobaric hypoxia on sea-level exercise: a cross-over study in humans. Eur J Appl Physiol, 88(4-5), 396–403. https://doi.org/10.1007/s00421-002-0708-z DOI: https://doi.org/10.1007/s00421-002-0708-z
Gore, C. J., Hahn, A. G., Aughey, R. J., Martin, D. T., Ashenden, M. J., Clark, S. A., . . . McKenna, M. J. (2001). Live high:train low increases muscle buffer capacity and submaximal cycling efficiency. Acta Physiol Scand, 173(3), 275–286. https://doi.org/10.1046/j.1365-201X.2001.00906.x DOI: https://doi.org/10.1046/j.1365-201X.2001.00906.x
Gupta, D. (2014). Role of Iron (Fe) in Body. IOSR Journal of Applied Chemistry, 7, 38–46. https://doi.org/10.9790/5736-071123846 DOI: https://doi.org/10.9790/5736-071123846
Schoene, R. B., Escourrou, P., Robertson, H. T., Nilson, K. L., Parsons, J. R., & Smith, N. J. (1983). Iron repletion decreases maximal exercise lactate concentrations in female athletes with minimal iron-deficiency anemia. J Lab Clin Med, 102(2), 306-312. https://www.ncbi.nlm.nih.gov/pubmed/6864076
Govus, A. D., Garvican-Lewis, L. A., Abbiss, C. R., Peeling, P., & Gore, C. J. (2015). Pre-Altitude Serum Ferritin Levels and Daily Oral Iron Supplement Dose Mediate Iron Parameter and Hemoglobin Mass Responses to Altitude Exposure. PLoS One, 10(8), e0135120. https://doi.org/10.1371/journal.pone.0135120 DOI: https://doi.org/10.1371/journal.pone.0135120
Ryan, B. J., Wachsmuth, N. B., Schmidt, W. F., Byrnes, W. C., Julian, C. G., Lovering, A. T., . . . Roach, R. C. (2014). AltitudeOmics: rapid hemoglobin mass alterations with early acclimatization to and de-acclimatization from 5260 m in healthy humans. PLoS One, 9(10), e108788. https://doi.org/10.1371/journal.pone.0108788 DOI: https://doi.org/10.1371/journal.pone.0108788
Friedmann, B., Jost, J., Rating, T., Weller, E., Werle, E., Eckardt, K. U., . . . Mairbaurl, H. (1999). Effects of iron supplementation on total body hemoglobin during endurance training at moderate altitude. Int J Sports Med, 20(2), 78–85. https://doi.org/10.1055/s-2007-971097 DOI: https://doi.org/10.1055/s-1999-970269
Nielsen, P., & Nachtigall, D. (1998). Iron supplementation in athletes. Current recommendations. Sports Med, 26(4), 207–216. https://doi.org/10.2165/00007256-199826040-00001 DOI: https://doi.org/10.2165/00007256-199826040-00001
T. D. P. Nandadeva, A. M. S. D. M. Dissanayake, A. A. J. Rajaratne, & S. D. I. Nanayakkara. (2019). Effect of iron supplementation during high altitude training on haemoglobin and iron status of Sri Lankan middle- and long-distance athletes. Sri Lanka Journal of Medicine, 28(1), 29. https://doi.org/10.4038/sljm.v28i1.114 DOI: https://doi.org/10.4038/sljm.v28i1.114
Chaeychomsri, W., Siruntawineti, J., Chaeychomsri, S., Hengsawadi, D., Cuptapan, Y., & Rungtaweechai, W. (2013, October 2013). Successful Development and Commercialization of Freeze-dried Crocodile Blood Product. The 39th Congress on Science and Technology of Thailand, Thailand.
Paratthakonkun, C., Vimuttipong, V., Nana, A., Chaijenkij, K., Soonthornworasiri, N., & Arthan, D. (2021). The Effects of Crocodile Blood Supplementation on Delayed-Onset Muscle Soreness. Nutrients, 13(7). https://doi.org/10.3390/nu13072312 DOI: https://doi.org/10.3390/nu13072312
Chook, C. Y. B., Chen, F. M., Leung, F. P., Chen, Z. Y., & Wong, W. T. (2021). Potential of crocodile blood as a medication and dietary supplement: A systemic review. Clin Exp Pharmacol Physiol. https://doi.org/10.1111/1440-1681.13524 DOI: https://doi.org/10.1111/1440-1681.13524
Ljungqvist, A., Jenoure, P. J., Engebretsen, L., Alonso, J. M., Bahr, R., Clough, A. F., . . . Dubi, C. (2009). The International Olympic Committee (IOC) consensus statement on periodic health evaluation of elite athletes, March 2009. Clin J Sport Med, 19(5), 347–365. https://doi.org/10.1097/JSM.0b013e3181b7332c DOI: https://doi.org/10.1097/JSM.0b013e3181b7332c
LaManca, J. J., & Haymes, E. M. (1993). Effects of iron repletion on VO2max, endurance, and blood lactate in women. Med Sci Sports Exerc, 25(12), 1386–1392. https://www.ncbi.nlm.nih.gov/pubmed/8107547 DOI: https://doi.org/10.1249/00005768-199312000-00012
Davies, K. J., Donovan, C. M., Refino, C. J., Brooks, G. A., Packer, L., & Dallman, P. R. (1984). Distinguishing effects of anemia and muscle iron deficiency on exercise bioenergetics in the rat. Am J Physiol, 246(6 Pt 1), E535–543. https://doi.org/10.1152/ajpendo.1984.246.6.E535 DOI: https://doi.org/10.1152/ajpendo.1984.246.6.E535
Davies, K. J., Maguire, J. J., Brooks, G. A., Dallman, P. R., & Packer, L. (1982). Muscle mitochondrial bioenergetics, oxygen supply, and work capacity during dietary iron deficiency and repletion. Am J Physiol, 242(6), E418–427. https://doi.org/10.1152/ajpendo.1982.242.6.E418 DOI: https://doi.org/10.1152/ajpendo.1982.242.6.E418
Schumacher, Y. O., Schmid, A., Grathwohl, D., Bultermann, D., & Berg, A. (2002). Hematological indices and iron status in athletes of various sports and performances. Med Sci Sports Exerc, 34(5), 869–875. https://doi.org/10.1097/00005768-200205000-00022 DOI: https://doi.org/10.1097/00005768-200205000-00022
Klingshirn, L. A., Pate, R. R., Bourque, S. P., Davis, J. M., & Sargent, R. G. (1992). Effect of iron supplementation on endurance capacity in iron-depleted female runners. Med Sci Sports Exerc, 24(7), 819–824. https://www.ncbi.nlm.nih.gov/pubmed/1501568 DOI: https://doi.org/10.1249/00005768-199207000-00013
Rosado-Perez, J., & Mendoza-Nunez, V. M. (2018). Relationship Between Aerobic Capacity With Oxidative Stress and Inflammation Biomarkers in the Blood of Older Mexican Urban-Dwelling Population. Dose Response, 16(2), 1559325818773000. https://doi.org/10.1177/1559325818773000 DOI: https://doi.org/10.1177/1559325818773000
Haase, V. H. (2010). Hypoxic regulation of erythropoiesis and iron metabolism. Am J Physiol Renal Physiol, 299(1), F1–13. https://doi.org/10.1152/ajprenal.00174.2010 DOI: https://doi.org/10.1152/ajprenal.00174.2010
Ramos-Campo, D. J., Martinez-Sanchez, F., Esteban-Garcia, P., Rubio-Arias, J. A., Clemente-Suarez, V. J., & Jimenez-Diaz, J. F. (2015). The effects of intermittent hypoxia training on hematological and aerobic performance in triathletes. Acta Physiol Hung, 102(4), 409–418. https://doi.org/10.1556/036.102.2015.4.8 DOI: https://doi.org/10.1556/036.102.2015.4.8
Wonnabussapawich, P., Hamlin, M. J., Lizamore, C. A., Manimmanakorn, N., Leelayuwat, N., Tunkamnerdthai, O., . . . Manimmanakorn, A. (2017). Living and Training at 825 m for 8 Weeks Supplemented With Intermittent Hypoxic Training at 3,000 m Improves Blood Parameters and Running Performance. J Strength Cond Res, 31(12), 3287–3294. https://doi.org/10.1519/JSC.0000000000002227 DOI: https://doi.org/10.1519/JSC.0000000000002227
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