Selenomethionine Supplementation Contributes to Reducing Oxidative Stress and Inflammation Markers following Exercise-Induced Muscle Damage
DOI:
https://doi.org/10.17309/tmfv.2024.3.15Keywords:
exercise, oxidative stress, inflammation, immune functionAbstract
Background. Exercise-induced muscle damage (EIMD) is a temporary response to intense or prolonged exercise that can cause muscle pain, inflammation, and impaired muscle function. Antioxidant supplementation is a proposed strategy to reduce EIMD symptoms by targeting reactive oxygen and nitrogen species (RONS) involved in the process.
Objective. The study aimed to investigate the effect of Selenomethionine supplementation on malondialdehyde (MDA) and C-reactive protein (CRP) levels resulting from Exercise-Induced Muscle Damage (EIMD).
Materials and methods. This study used a randomized pretest-posttest control group design, involving a total of 32 male recreational students from the State University of Surabaya (Indonesian: Universitas Negeri Surabaya), (age 19.25 ± 0.76 years, body mass 63.16 ± 3.38 kg, height 167.28 ± 4.54 cm, body fat 19.60% ± 4.57%). The participants were randomly assigned to the selenomethionine group (SEM, 100 µg/day) or placebo group (PLA, corn starch 100 mg/day) for a period of 28 days (4 weeks). On days 1 (baseline) and 29, participants underwent a single bout of EIMD. Blood samples were collected 24 hours post-EIMD to measure MDA and CRP concentrations in plasma. The statistical analysis was conducted using paired sample t-test.
Results. The placebo group experienced a significant increase in plasma MDA and CRP concentrations after EIMD compared with baseline values (p < 0.05). However, the SeMet group showed lower plasma MDA and CRP levels than the placebo group.
Conclusions. Daily Selenomethionine supplementation for 28 days has been found to reduce oxidative stress by lowering MDA levels in the blood and to decrease inflammation by reducing CRP levels post-exercise-induced muscle damage. This indicates a lower risk of EIMD due to reduced oxidative stress and inflammation.
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Irawan, R., Mahmudiono, T., & Martiana, T. (2021). Interleukin-6 as Immune System and Inflammation Biomarker on the Response of Basic Pencak Silat Exercise in Perguruan Pencak Silat Perisai Diri, Bojonegoro. Open Access Macedonian Journal of Medical Sciences, 9(T6), 179–183. https://doi.org/10.3889/oamjms.2021.7303 DOI: https://doi.org/10.3889/oamjms.2021.7303
Irawan, R. J., Sulistyarto, S., & Rimawati, N. (2024). Kencur Supplementation for Attenuating Exercise-Induced Muscle Damage and Delayed-Onset Muscle Soreness. Kemas, 19(3), 438-446. https://doi.org/10.15294/kemas.v19i3.42151
Fernández-Lázaro, D., Mielgo-Ayuso, J., Seco Calvo, J., Córdova Martínez, A., Caballero García, A., & Fernandez-Lazaro, C. (2020). Modulation of Exercise-Induced Muscle Damage, Inflammation, and Oxidative Markers by Curcumin Supplementation in a Physically Active Population: A Systematic Review. Nutrients, 12(2), 501. https://doi.org/10.3390/nu12020501 DOI: https://doi.org/10.3390/nu12020501
Kong, P. W., Chua, Y. H., Kawabata, M., Burns, S. F., & Cai, C. (2018). Effect of Post-Exercise Massage on Passive Muscle Stiffness Measured Using Myotonometry - A Double-Blind Study. Journal of Sports Science & Medicine, 17(4), 599-606.
Nanavati, K., Rutherfurd-Markwick, K., Lee, S. J., Bishop, N. C., & Ali, A. (2022). Effect of curcumin supplementation on exercise-induced muscle damage: a narrative review. European Journal of Nutrition, 61(8), 3835-3855. https://doi.org/10.1007/s00394-022-02943-7 DOI: https://doi.org/10.1007/s00394-022-02943-7
Souissi, W., Bouzid, M. A., Farjallah, M. A., Ben Mahmoud, L., Boudaya, M., Engel, F. A., & Sahnoun, Z. (2020). Effect of Different Running Exercise Modalities on Post-Exercise Oxidative Stress Markers in Trained Athletes. International Journal of Environmental Research and Public Health, 17(10), 3729. https://doi.org/10.3390/ijerph17103729 DOI: https://doi.org/10.3390/ijerph17103729
Suzuki, K., Tominaga, T., Ruhee, R. T., & Ma, S. (2020). Characterization and Modulation of Systemic Inflammatory Response to Exhaustive Exercise in Relation to Oxidative Stress. Antioxidants, 9(5), 401. https://doi.org/10.3390/antiox9050401 DOI: https://doi.org/10.3390/antiox9050401
Bazzucchi, I., Patrizio, F., Ceci, R., Duranti, G., Sgrò, P., Sabatini, S., Di Luigi, L., Sacchetti, M., & Felici, F. (2019). The Effects of Quercetin Supplementation on Eccentric Exercise-Induced Muscle Damage. Nutrients, 11(1), 205. https://doi.org/10.3390/nu11010205 DOI: https://doi.org/10.3390/nu11010205
Del Giudice, M., & Gangestad, S. W. (2018). Rethinking IL-6 and CRP: Why they are more than inflammatory biomarkers, and why it matters. Brain, Behavior, and Immunity, 70, 61-75. https://doi.org/10.1016/j.bbi.2018.02.013 DOI: https://doi.org/10.1016/j.bbi.2018.02.013
Ammar, A., Trabelsi, K., Boukhris, O., Glenn, J., Bott, N., Masmoudi, L., Hakim, A., Chtourou, H., Driss, T., Hoekelmann, A., & El Abed, K. (2020). Effects of Aerobic-, Anaerobic- and Combined-Based Exercises on Plasma Oxidative Stress Biomarkers in Healthy Untrained Young Adults. International Journal of Environmental Research and Public Health, 17(7), 2601. https://doi.org/10.3390/ijerph17072601 DOI: https://doi.org/10.3390/ijerph17072601
Matta, L., Fonseca, T. S., Faria, C. C., Lima-Junior, N. C., De Oliveira, D. F., Maciel, L., Boa, L. F., Pierucci, A. P. T. R., Ferreira, A. C. F., Nascimento, J. H. M., Carvalho, D. P., & Fortunato, R. S. (2021). The Effect of Acute Aerobic Exercise on Redox Homeostasis and Mitochondrial Function of Rat White Adipose Tissue. Oxidative Medicine and Cellular Longevity, 2021, 1-15. https://doi.org/10.1155/2021/4593496 DOI: https://doi.org/10.1155/2021/4593496
O’Connor, E., Mündel, T., & Barnes, M. J. (2022). Nutritional Compounds to Improve Post-Exercise Recovery. Nutrients, 14(23), 5069. https://doi.org/10.3390/nu14235069 DOI: https://doi.org/10.3390/nu14235069
Suzuki, K. (2018). Cytokine response to exercise and its modulation. Antioxidants, 7(1), 17. https://doi.org/10.3390/antiox7010017 DOI: https://doi.org/10.3390/antiox7010017
Arazi, H., Eghbali, E., & Suzuki, K. (2021). Creatine Supplementation, Physical Exercise and Oxidative Stress Markers: A Review of the Mechanisms and Effectiveness. Nutrients, 13(3), 869. https://doi.org/10.3390/nu13030869 DOI: https://doi.org/10.3390/nu13030869
Cicchella, A., Stefanelli, C., & Massaro, M. (2021). Upper Respiratory Tract Infections in Sport and the Immune System Response. A Review. Biology, 10(5), 362. https://doi.org/10.3390/biology10050362 DOI: https://doi.org/10.3390/biology10050362
Tidball, J. G., & Villalta, S. A. (2010). Regulatory interactions between muscle and the immune system during muscle regeneration. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 298(5), R1173–R1187. https://doi.org/10.1152/ajpregu.00735.2009 DOI: https://doi.org/10.1152/ajpregu.00735.2009
Ruhee, R. T., Ma, S., & Suzuki, K. (2020). Protective Effects of Sulforaphane on Exercise-Induced Organ Damage via Inducing Antioxidant Defense Responses. Antioxidants, 9(2), 136. https://doi.org/10.3390/antiox9020136 DOI: https://doi.org/10.3390/antiox9020136
Wadley, A. J., Keane, G., Cullen, T., James, L., Vautrinot, J., Davies, M., Hussey, B., Hunter, D. J., Mastana, S., Holliday, A., Petersen, S. V., Bishop, N. C., Lindley, M. R., & Coles, S. J. (2019). Characterization of extracellular redox enzyme concentrations in response to exercise in humans. Journal of Applied Physiology, 127(3), 858-866. https://doi.org/10.1152/japplphysiol.00340.2019 DOI: https://doi.org/10.1152/japplphysiol.00340.2019
Kyriakidou, Y., Wood, C., Ferrier, C., Dolci, A., & Elliott, B. (2021). The effect of Omega-3 polyunsaturated fatty acid supplementation on exercise-induced muscle damage. Journal of the International Society of Sports Nutrition, 18(1). https://doi.org/10.1186/s12970-020-00405-1 DOI: https://doi.org/10.1186/s12970-020-00405-1
Lee, M.-C., Jhang, W.-L., Lee, C.-C., Kan, N.-W., Hsu, Y.-J., Ho, C.-S., Chang, C.-H., Cheng, Y.-C., Lin, J.-S., & Huang, C.-C. (2021). The Effect of Kefir Supplementation on Improving Human Endurance Exercise Performance and Antifatigue. Metabolites, 11(3), 136. https://doi.org/10.3390/metabo11030136 DOI: https://doi.org/10.3390/metabo11030136
Ellingsgaard, H., Hojman, P., & Pedersen, B. K. (2019). Exercise and health — emerging roles of IL-6. Current Opinion in Physiology, 10, 49–54. https://doi.org/10.1016/J.COPHYS.2019.03.009 DOI: https://doi.org/10.1016/j.cophys.2019.03.009
Irawan, R. J., Sulistyarto, S., & Rimawati, N. (2022). Suplementasi Ekstrak Kencur (Kaempferia Galanga linn) terhadap Kadar Plasma Malondealdehide (MDA) dan Interleukin-6 (IL-6) Pasca Aktivitas Latihan Aerobik Supplementation of Kencur (Kaempferia Galanga Linn) Extract on Malondealdehyde (MDA) and Interleukin-6 (IL-6) Plasma Levels Post Aerobic Training Activity. Amerta Nutrition , 6(1SP), 140-145. https://doi.org/10.20473/amnt.v6i1SP.2022.140 DOI: https://doi.org/10.20473/amnt.v6i1SP.2022.140-145
He, F., Chuang, C.-C., Zhou, T., Jiang, Q., Sedlock, D. A., & Zuo, L. (2018). Redox correlation in muscle lengthening and immune response in eccentric exercise. PLOS ONE, 13(12), e0208799. https://doi.org/10.1371/journal.pone.0208799 DOI: https://doi.org/10.1371/journal.pone.0208799
Martinez-Ferran, M., Sanchis-Gomar, F., Lavie, C. J., Lippi, G., & Pareja-Galeano, H. (2020). Do Antioxidant Vitamins Prevent Exercise-Induced Muscle Damage? A Systematic Review. Antioxidants, 9(5), 372. https://doi.org/10.3390/antiox9050372 DOI: https://doi.org/10.3390/antiox9050372
Brendler, T., Al‐Harrasi, A., Bauer, R., Gafner, S., Hardy, M. L., Heinrich, M., Hosseinzadeh, H., Izzo, A. A., Michaelis, M., Nassiri‐Asl, M., Panossian, A., Wasser, S. P., & Williamson, E. M. (2021). Botanical drugs and supplements affecting the immune response in the time of COVID ‐19: Implications for research and clinical practice. Phytotherapy Research, 35(6), 3013-3031. https://doi.org/10.1002/ptr.7008 DOI: https://doi.org/10.1002/ptr.7008
Righi, N. C., Schuch, F. B., De Nardi, A. T., Pippi, C. M., de Almeida Righi, G., Puntel, G. O., da Silva, A. M. V., & Signori, L. U. (2020). Effects of vitamin C on oxidative stress, inflammation, muscle soreness, and strength following acute exercise: meta-analyses of randomized clinical trials. European Journal of Nutrition, 59(7), 2827-2839. https://doi.org/10.1007/s00394-020-02215-2 DOI: https://doi.org/10.1007/s00394-020-02215-2
Wang, I.-L., Hsiao, C.-Y., Li, Y.-H., Meng, F.-B., Huang, C.-C., & Chen, Y.-M. (2019). Nanobubbles Water Curcumin Extract Reduces Injury Risks on Drop Jumps in Women: A Pilot Study. Evidence-Based Complementary and Alternative Medicine, 2019, 1-9. https://doi.org/10.1155/2019/8647587 DOI: https://doi.org/10.1155/2019/8647587
Lima, L. C. R., Barreto, R. V., Bassan, N. M., Greco, C. C., & Denadai, B. S. (2019). Consumption of An Anthocyanin-Rich Antioxidant Juice Accelerates Recovery of Running Economy and Indirect Markers of Exercise-Induced Muscle Damage Following Downhill Running. Nutrients, 11(10), 2274. https://doi.org/10.3390/nu11102274 DOI: https://doi.org/10.3390/nu11102274
Massaro, M., Scoditti, E., Carluccio, M., Kaltsatou, A., & Cicchella, A. (2019). Effect of Cocoa Products and Its Polyphenolic Constituents on Exercise Performance and Exercise-Induced Muscle Damage and Inflammation: A Review of Clinical Trials. Nutrients, 11(7), 1471. https://doi.org/10.3390/nu11071471 DOI: https://doi.org/10.3390/nu11071471
Dos Reis, T. M. P., Aguiar, G. G., de Azevedo, L. P., Silva Lima, E., André Dellagrana, R., & Rossato, M. (2023). Effects of acai supplementation (Euterpe precatoria Mart) on muscle recovery markers after jump protocol. Research in Sports Medicine, 1-17. https://doi.org/10.1080/15438627.2023.2189114 DOI: https://doi.org/10.1080/15438627.2023.2189114
Tanabe, Y., Fujii, N., & Suzuki, K. (2021). Dietary Supplementation for Attenuating Exercise-Induced Muscle Damage and Delayed-Onset Muscle Soreness in Humans. Nutrients, 14(1), 70. https://doi.org/10.3390/nu14010070 DOI: https://doi.org/10.3390/nu14010070
Owens, D. J., Twist, C., Cobley, J. N., Howatson, G., & Close, G. L. (2019). Exercise-induced muscle damage: What is it, what causes it and what are the nutritional solutions? European Journal of Sport Science, 19(1), 71-85. https://doi.org/10.1080/17461391.2018.1505957 DOI: https://doi.org/10.1080/17461391.2018.1505957
Sánchez Díaz, M., Martín-Castellanos, A., Fernández-Elías, V. E., López Torres, O., & Lorenzo Calvo, J. (2022). Effects of Polyphenol Consumption on Recovery in Team Sport Athletes of Both Sexes: A Systematic Review. Nutrients, 14(19), 4085. https://doi.org/10.3390/nu14194085 DOI: https://doi.org/10.3390/nu14194085
Kruk, J., Aboul-Enein, B. H., & Duchnik, E. (2021). Exercise-induced oxidative stress and melatonin supplementation: current evidence. The Journal of Physiological Sciences, 71(1), 27. https://doi.org/10.1186/s12576-021-00812-2 DOI: https://doi.org/10.1186/s12576-021-00812-2
Thirupathi, A., Wang, M., Lin, J. K., Fekete, G., István, B., Baker, J. S., & Gu, Y. (2021). Effect of Different Exercise Modalities on Oxidative Stress: A Systematic Review. BioMed Research International, 2021, 1-10. https://doi.org/10.1155/2021/1947928 DOI: https://doi.org/10.1155/2021/1947928
Amalraj, A., Divya, C., & Gopi, S. (2020). The Effects of Bioavailable Curcumin (Cureit) on Delayed Onset Muscle Soreness Induced By Eccentric Continuous Exercise: A Randomized, Placebo-Controlled, Double-Blind Clinical Study. Journal of Medicinal Food, 23(5), 545-553. https://doi.org/10.1089/jmf.2019.4533 DOI: https://doi.org/10.1089/jmf.2019.4533
Powers, S. K., Deminice, R., Ozdemir, M., Yoshihara, T., Bomkamp, M. P., & Hyatt, H. (2020). Exercise-induced oxidative stress: Friend or foe? Journal of Sport and Health Science, 9(5), 415–425. https://doi.org/10.1016/j.jshs.2020.04.001 DOI: https://doi.org/10.1016/j.jshs.2020.04.001
Guerrero, C., Collado-Boira, E., Martinez-Navarro, I., Hernando, B., Hernando, C., Balino, P., & Muriach, M. (2021). Impact of Plasma Oxidative Stress Markers on Post-race Recovery in Ultramarathon Runners: A Sex and Age Perspective Overview. Antioxidants, 10(3), 355. https://doi.org/10.3390/antiox10030355 DOI: https://doi.org/10.3390/antiox10030355
Lin, C.-H., Lin, Y.-A., Chen, S.-L., Hsu, M.-C., & Hsu, C.-C. (2021). American Ginseng Attenuates Eccentric Exercise-Induced Muscle Damage via the Modulation of Lipid Peroxidation and Inflammatory Adaptation in Males. Nutrients, 14(1), 78. https://doi.org/10.3390/nu14010078 DOI: https://doi.org/10.3390/nu14010078
Williamson, J., & Davison, G. (2020). Targeted Antioxidants in Exercise-Induced Mitochondrial Oxidative Stress: Emphasis on DNA Damage. Antioxidants, 9(11), 1142. https://doi.org/10.3390/antiox9111142 DOI: https://doi.org/10.3390/antiox9111142
Lior, O., Sklerovsy-Benjaminov, F., Lish, I., Konokoff, F., & Naftali, T. (2019). Treatment of Irritable Bowel Syndrome with a Combination of Curcumin, Green Tea and Selenomethionine Has a Positive Effect on Satisfaction with Bowel Habits. Journal of Biosciences and Medicines, 07(05), 170-179. https://doi.org/10.4236/jbm.2019.75018 DOI: https://doi.org/10.4236/jbm.2019.75018
Mal’tseva, V. N., Goltyaev, M. V., Turovsky, E. A., & Varlamova, E. G. (2022). Immunomodulatory and Anti-Inflammatory Properties of Selenium-Containing Agents: Their Role in the Regulation of Defense Mechanisms against COVID-19. International Journal of Molecular Sciences, 23(4), 2360. https://doi.org/10.3390/ijms23042360 DOI: https://doi.org/10.3390/ijms23042360
Goldsztejn, G., Mundlapati, V. R., Brenner, V., Gloaguen, E., & Mons, M. (2022). Selenium in Proteins: Conformational Changes Induced by Se Substitution on Methionine, as Studied in Isolated Model Peptides by Optical Spectroscopy and Quantum Chemistry. Molecules, 27(10), 3163. https://doi.org/10.3390/molecules27103163 DOI: https://doi.org/10.3390/molecules27103163
Bjørklund, G., Shanaida, M., Lysiuk, R., Antonyak, H., Klishch, I., Shanaida, V., & Peana, M. (2022). Selenium: An Antioxidant with a Critical Role in Anti-Aging. Molecules, 27(19), 6613. https://doi.org/10.3390/molecules27196613 DOI: https://doi.org/10.3390/molecules27196613
Weaver, K., & Skouta, R. (2022). The Selenoprotein Glutathione Peroxidase 4: From Molecular Mechanisms to Novel Therapeutic Opportunities. Biomedicines, 10(4), 891. https://doi.org/10.3390/biomedicines10040891 DOI: https://doi.org/10.3390/biomedicines10040891
Cerqueira, É., Marinho, D. A., Neiva, H. P., & Lourenço, O. (2020). Inflammatory Effects of High and Moderate Intensity Exercise—A Systematic Review. Frontiers in Physiology, 10. https://doi.org/10.3389/fphys.2019.01550 DOI: https://doi.org/10.3389/fphys.2019.01550
Costello, J. T., Rendell, R. A., Furber, M., Massey, H. C., Tipton, M. J., Young, J. S., & Corbett, J. (2018). Effects of acute or chronic heat exposure, exercise and dehydration on plasma cortisol, IL-6 and CRP levels in trained males. Cytokine, 110, 277-283. https://doi.org/10.1016/j.cyto.2018.01.018 DOI: https://doi.org/10.1016/j.cyto.2018.01.018
Kawamura, T., & Muraoka, I. (2018). Exercise-Induced Oxidative Stress and the Effects of Antioxidant Intake from a Physiological Viewpoint. Antioxidants, 7(9), 119. https://doi.org/10.3390/antiox7090119 DOI: https://doi.org/10.3390/antiox7090119
Boukhris, O., Trabelsi, K., Abdessalem, R., Hsouna, H., Ammar, A., Glenn, J. M., Bott, N., Irandoust, K., Taheri, M., Turki, M., Ayadi, F., Bragazzi, N. L., Engel, F. A., & Chtourou, H. (2020). Effects of the 5-m Shuttle Run Test on Markers of Muscle Damage, Inflammation, and Fatigue in Healthy Male Athletes. International Journal of Environmental Research and Public Health, 17(12), 4375. https://doi.org/10.3390/ijerph17124375 DOI: https://doi.org/10.3390/ijerph17124375
Heiss, R., Lutter, C., Freiwald, J., Hoppe, M., Grim, C., Poettgen, K., Forst, R., Bloch, W., Hüttel, M., & Hotfiel, T. (2019). Advances in Delayed-Onset Muscle Soreness (DOMS) – Part II: Treatment and Prevention. Sportverletzung · Sportschaden, 33(01), 21-29. https://doi.org/10.1055/a-0810-3516 DOI: https://doi.org/10.1055/a-0810-3516
Taherkhani, S., Suzuki, K., & Castell, L. (2020). A Short Overview of Changes in Inflammatory Cytokines and Oxidative Stress in Response to Physical Activity and Antioxidant Supplementation. Antioxidants, 9(9), 886. https://doi.org/10.3390/antiox9090886 DOI: https://doi.org/10.3390/antiox9090886
Prabhu, K. S., Zamamiri-Davis, F., Stewart, J. B., Thompson, J. T., Sordillo, L. M., & Reddy, C. C. (2002). Selenium deficiency increases the expression of inducible nitric oxide synthase in RAW 264.7 macrophages: role of nuclear factor-κB in up-regulation. Biochemical Journal, 366(1), 203–209. https://doi.org/10.1042/bj20020256 DOI: https://doi.org/10.1042/bj20020256
Maehira, F., Miyagi, I., & Eguchi, Y. (2003). Selenium regulates transcription factor NF-κB activation during the acute phase reaction. Clinica Chimica Acta, 334(1-2), 163-171. https://doi.org/10.1016/S0009-8981(03)00223-7 DOI: https://doi.org/10.1016/S0009-8981(03)00223-7
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